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Anti-ATM Antibody

Rabbit monoclonal antibody to ATM from ABclonal (A10745).
Name: Anti-ATM Antibody
Description: Rabbit monoclonal antibody to ATM.
Applications: WB, IHC, IF
Dilutions: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, ICC: 1:50 - 1:200, IF: 1:50 - 1:100.
Reactivity: Human
Immunogen: Recombinant protein of human ATM.
Host: Rabbit
Clonality: Monoclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.
Tissue Specificity: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
Involvement in Disease: Ataxia telangiectasia: A rare recessive disorder characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. Patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
Sequence Similarities: Belongs to the PI3/PI4-kinase family. ATM subfamily.
Post-Translational Modification: Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
Cellular Location: Nucleus. Cytoplasmic vesicle.

Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
UniProt: Q13315
Gene ID: 472
Synonyms: A-T mutated Antibody
A-T mutated homolog Antibody
AT mutated Antibody
AT1 Antibody
ATA Antibody
Ataxia telangiectasia mutated Antibody
Ataxia telangiectasia mutated gene Antibody
Ataxia telangiectasia mutated homolog Antibody
Ataxia telangiectasia mutated homolog (human) Antibody
ATC Antibody
ATD Antibody
ATDC Antibody
ATE Antibody
ATM serine/threonine kinase Antibody
ATM_HUMAN Antibody
DKFZp781A0353 Antibody
MGC74674 Antibody
OTTHUMP00000232981 Antibody
Serine protein kinase ATM Antibody
Serine-protein kinase ATM Antibody
Serine/threonine-protein kinase ATM Antibody
Tefu Antibody
TEL1 Antibody
TEL1, telomere maintenance 1, homolog Antibody
TELO1 Antibody
Telomere fusion protein Antibody
Information: Target information shown above is from the UniProt Consortium.
Western blot analysis of extracts of CRC cells, using ATM antibody.
Immunohistochemistry of paraffin-embedded human pancreas using ATM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using ATM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ATM antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cell using ATM antibody.
Immunofluorescence analysis of CRC cell using ATM antibody.
Immunofluorescence analysis of Hela cell using ATM antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.
Description: Rabbit monoclonal antibody to ATM
Application: WB, ICC/IF, IHC
Host: Rabbit
Reactivity: Human
Immunogen: Recombinant antibody.

£269 – £400

Total Citations (4)
Description: Rabbit polyclonal antibody to ATM (Phospho-Ser1981)
Application: WB, IHC
Host: Rabbit
Reactivity: Human
Immunogen: Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.

£180 – £234

Description: Rabbit polyclonal antibody to Phospho-ATM-S1981.
Application: WB, IHC, IF
Host: Rabbit
Isotype: IgG
Reactivity: Human

£199 – £452

Total Citations (2)
Description: Rabbit polyclonal antibody to ATM (I1987)
Application: IHC
Host: Rabbit
Reactivity: Human
Immunogen: Synthetic peptide, corresponding to amino acids 1957-2006 of Human ATM.

£165 – £283

Please Note: Anti-ATM Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A10745
Size Price Quantity
100µl £372
200µl £565

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Lead Time: Currently 4-6 Days
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