Donkey Anti-Guinea Pig IgG H&L Antibody (CF®660C), Cross-Adsorbed (A343857)

Normalized absorption and emission spectra of CF®660C (solid lines) and CF®660R (dashed lines) goat anti-mouse antibody conjugates measured in PBS.

CF®660 exhibits exceptional photostability, and CF®660C is more photostable than Alexa Fluor® 660. HeLa cells were stained with mouse anti-tubulin primary antibody followed by goat anti-mouse secondary antibodies conjugated to the indicated dyes. Cells were continuously illuminated using a mercury arc lamp with a Cy®5 filter set, with images acquired every 10 seconds for 5 minutes. Average fluorescence intensity was normalized to the initial time point.

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.