Goat Anti-Guinea Pig IgG H&L Antibody (CF®555), Cross-Adsorbed (A343943)

Normalized absorption and emission spectra of a CF®555 goat anti-mouse antibody conjugate measured in PBS.

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.

HeLa cells stained with rabbit anti-COX IV followed by CF®555 goat anti-rabbit to label mitochondria (red), mouse anti-Golgin-97 followed by CF®488A goat anti-mouse to label the Golgi complex (green), and CF®640R phalloidin to visualize actin filaments (cyan).

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.