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Anti-FEN-1 Antibody

Rabbit polyclonal antibody to FEN-1 from ABclonal (A0129).
Name: Anti-FEN-1 Antibody
Description: Rabbit polyclonal antibody to FEN-1.
Applications: WB, IF
Dilutions: WB: 1:500 - 1:1000, IF: 1:20 - 1:50.
Reactivity: Human
Immunogen: A synthetic peptide of human FEN1.
Protein Length: 380
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Sequence Similarities: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Post-Translational Modification: Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Cellular Location: Nucleus > Nucleolus. Nucleus > Nucleoplasm.

Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
UniProt: P39748
Gene ID: 2237
Synonyms: DNase IV Antibody
FEN1 Antibody
FEN1_HUMAN Antibody
Flap endonuclease 1 Antibody
Flap structure specific endonuclease 1 Antibody
Flap structure-specific endonuclease 1 Antibody
hFEN-1 Antibody
hFEN1 Antibody
Maturation factor 1 Antibody
MF1 Antibody
Rad2 Antibody
Information: Target information shown above is from the UniProt Consortium.
Western blot analysis of extracts of HepG2 cells, using FEN1 antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.
Description: Rabbit polyclonal antibody to FEN1.
Application: WB, IHC, IF
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£148 – £372

Description: Rabbit polyclonal antibody to FEN1
Application: WB, IHC, IF
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthesized peptide derived from internal of human FEN1.

£180 – £234

Description: Rabbit polyclonal antibody to FEN-1
Application: WB, IHC, IF, ELISA
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the Internal region of human FEN-1.

£180 – £234

Description: Mouse monoclonal antibody to FEN1 (7H8)
Application: WB, ICC
Host: Mouse
Reactivity: Human, Mouse, Rat
Immunogen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.

£220 – £366

Please Note: Anti-FEN-1 Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A0129
Size Price Quantity
100µl £243
200µl £372

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Lead Time: Currently 4-6 Days
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe