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Anti-Fox3/NeuN Antibody

 
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Total Citations (42)
Mouse monoclonal (1B7) antibody to Fox3 from EnCor Biotechnology (MCA-1B7-AP).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab104224
Name: Anti-Fox3/NeuN Antibody
Description: Mouse monoclonal (1B7) antibody to Fox3.
Applications: WB, ICC/IF, IHC
Dilutions: WB: 1:1,000-1:2,000. ICC/IF and IHC: 1:1,000.
Immunogen: N-terminal 100 amino acids of human Fox3 expressed in and purified from E. coli.
Host: Mouse
Clonality: Monoclonal
Clone: 1B7
Isotype: IgG2a
Conjugate: Unconjugated
Purification: Immunogen affinity purified.
Concentration: 1 mg/ml
Molecular Weight: 46, 48 kDa
Product Form: Liquid
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Introduction: Fox3 is one of a family of mammalian homologues of Fox-1, which was originally discovered in C. elegans as a gene involved in sex determination. Fox is an acronym of “Feminizing locus on X”. The mammalian genome contains three genes homologous to C. elegans Fox-1, usually called Fox1, Fox2 and Fox3. All these Fox proteins are about 46 kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. This motif corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. An alternate name for Fox3 is hexaribonucleotide binding protein 3, and the Fox proteins are believed to have a role in the regulation of mRNA splicing. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN was first described in 1994 by Mullen et al. (2), who raised a series of monoclonal antibodies to mouse antigens with the original intent of finding mouse species specific markers useful for transplantation experiments. In the event they obtained a clone, called mAb A60, which proved to bind an antigen expressed only in neuronal nuclei and to a lesser extent the cytoplasm of neuronal cells, and which appeared to work on all vertebrates. A few neuronal cell types were not recognized by the NeuN antibody, such as cerebellar Purkinje cells, olfactory Mitral cells and retinal photoreceptors. However, the vast majority of neurons are strongly NeuN positive, and NeuN immunoreactivity has been widely used to measure the neuron/glial ratio in brain regions (3). The protein bound by this antibody was not characterized, though the molecular weight of this protein was shown to be closely spaced bands running at 46-48 kDa on SDS-PAGE gels. The exact identity of the NeuN protein was not elucidated in this paper nor in several attempts that followed for many years later. Despite this the mAb A66 antibody has become very widely used as a robust marker of neurons and neuronal stem cells, and a recent Medline search using the keyword “neun” produced over 1,100 hits. Recently Kim et al. used proteomic methods to show that NeuN corresponds to Fox3 (4). NeuN/Fox-3 is therefore a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as the immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal regions of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated. For a review of the Fox family of proteins see reference 5. The HGNC name for this protein is RBFOX3.
Synonyms: FLJ56884 Antibody
FLJ58356 Antibody
Fox-1 homolog C Antibody
fox1 homolog C Antibody
FOX3NeuN Antibody
hexaribonucleotide binding protein 3 Antibody
HRNBP3 Antibody
NeuN Antibody
neuronal nuclei Antibody
Rbfox3 Antibody
RFOX3_HUMAN Antibody
RNA binding protein fox-1 homolog 3 Antibody
RNA binding protein, fox 1 homolog (C. elegans) 3 Antibody
Immunohistochemisty - Anti-Fox3/NeuN Antibody from EnCor Biotechnology (MCA-1B7-AP) - Antibodies.com
Formalin fixed rat brain stem neurons stained with MCA-1B7-AP to NeuN in green and counter stained with CPCA-MAP2, our chicken polyclonal antibody to microtubule associated protein 2 in red. The nuclei of cells are revealed with DAPI in blue. The MCA-1B7-AP antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of other cell types. The MAP2 antibody binds to dendrites and overlaps with Fox3 staining in perikarya. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells. Note that we have used the same immunogen to generate a rabbit polyclonal antibody to Fox3/NeuN, RPCA-Fox3. 
Western Blot - Anti-Fox3/NeuN Antibody from EnCor Biotechnology (MCA-1B7-AP) - Antibodies.com
Blots of crude rat brain extract stained with MCA-1B7-AP. Fox3/NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48 kDa.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

Mucopolysaccharidosis IIIB confers enhanced neonatal intracranial transduction by AAV8 but not by 5, 9 or rh10.

Detrimental role of the EP1 prostanoid receptor in blood-brain barrier damage following experimental ischemic stroke.

Pain in experimental autoimmune encephalitis: a comparative study between different mouse models.

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites ab104224

Yang Y  et al. miR-16 and Fluoxetine Both Reverse Autophagic and Apoptotic Change in Chronic Unpredictable Mild Stress Model Rats. Front Neurosci 11:428 (2017).

Cites ab104224

Ahlfeld J  et al. Neurogenesis from Sox2 expressing cells in the adult cerebellar cortex. Sci Rep 7:6137 (2017).

Cites ab104224

Bi RY  et al. Estradiol upregulates voltage-gated sodium channel 1.7 in trigeminal ganglion contributing to hyperalgesia of inflamed TMJ. PLoS One 12:e0178589 (2017).

Cites ab104224

Wei X  et al. Impaired Spinal Glucocorticoid Receptor Signaling Contributes to the Attenuating Effect of Depression on Mechanical Allodynia and Thermal Hyperalgesia in Rats with Neuropathic Pain. Front Cell Neurosci 11:145 (2017).

Cites ab104224

Sun Y  et al. Therapeutic effect of apocynin through antioxidant activity and suppression of apoptosis and inflammation after spinal cord injury. Exp Ther Med 13:952-960 (2017).

Cites ab104224

Satoh JI  et al. Expression of GPR17, a regulator of oligodendrocyte differentiation and maturation, in Nasu-Hakola disease brains. Intractable Rare Dis Res 6:50-54 (2017).

Cites ab104224

Yamanishi E  et al. A novel form of necrosis, TRIAD, occurs in human Huntington's disease. Acta Neuropathol Commun 5:19 (2017).

Cites ab104224

Tang WC  et al. Early electroacupuncture treatment ameliorates neuroinflammation in rats with traumatic brain injury. BMC Complement Altern Med 16:470 (2016).

Cites ab104224

Ishikawa M  et al. 24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure. Sci Rep 6:33886 (2016).

Cites ab104224

Wang Z  et al. Transient receptor potential channel 1/4 reduces subarachnoid hemorrhage-induced early brain injury in rats via calcineurin-mediated NMDAR and NFAT dephosphorylation. Sci Rep 6:33577 (2016).

Cites ab104224

Waung MW  et al. Metabotropic glutamate receptor 5: a target for migraine therapy. Ann Clin Transl Neurol 3:560-71 (2016).

Cites ab104224

Schmidt AF  et al. Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. J Neuroinflammation 13:238 (2016).

Cites ab104224

Xu Y  et al. RIP3 induces ischemic neuronal DNA degradation and programmed necrosis in rat via AIF. Sci Rep 6:29362 (2016).

Cites ab104224

Sano K  et al. Pubertal activation of estrogen receptor a in the medial amygdala is essential for the full expression of male social behavior in mice. Proc Natl Acad Sci U S A 113:7632-7 (2016).

Cites ab104224

Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016).

Cites ab104224

Xu Y  et al. Mechanisms of PDGF siRNA-mediated inhibition of bone cancer pain in the spinal cord. Sci Rep 6:27512 (2016).

Cites ab104224

Hirako S  et al. Antenatal Saireito (TJ-114) Can Improve Pulmonary Hypoplasia and Pulmonary Vascular Remodeling in Nitrofen-Induced Congenital Diaphragmatic Hernia. Phytother Res 30:1474-80 (2016).

Cites ab104224

Vasilev DS  et al. Prenatal Hypoxia in Different Periods of Embryogenesis Differentially Affects Cell Migration, Neuronal Plasticity, and Rat Behavior in Postnatal Ontogenesis. Front Neurosci 10:126 (2016).

Cites ab104224

Abdul Rahman NZ  et al. Mitogen-Activated Protein Kinase Phosphatase-2 Deletion Impairs Synaptic Plasticity and Hippocampal-Dependent Memory. J Neurosci 36:2348-54 (2016).

Cites ab104224

Zhu JW  et al. Toll-Like Receptor 4 Deficiency Impairs Motor Coordination. Front Neurosci 10:33 (2016).

Cites ab104224

Zhang W  et al. Hyperactive somatostatin interneurons contribute to excitotoxicity in neurodegenerative disorders. Nat Neurosci 19:557-559 (2016).

Cites ab104224

Chavan R  et al. Liver-derived ketone bodies are necessary for food anticipation. Nat Commun 7:10580 (2016).

Cites ab104224

Johansson MM  et al. Spatial sexual dimorphism of X and Y homolog gene expression in the human central nervous system during early male development. Biol Sex Differ 7:5 (2016).

Cites ab104224

García-Castro IL  et al. Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical Neurons. PLoS One 10:e0146082 (2015).

Cites ab104224

Baarine M  et al. Functional Characterization of IPSC-Derived Brain Cells as a Model for X-Linked Adrenoleukodystrophy. PLoS One 10:e0143238 (2015).

Cites ab104224

Lim S  et al. dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis. Nat Commun 6:8244 (2015).

Cites ab104224

Ebbers L  et al. L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei. J Biol Chem 290:23692-710 (2015).

Cites ab104224

Kim J  et al. High-capacity glycolytic and mitochondrial oxidative metabolisms mediate the growth ability of glioblastoma. Int J Oncol 47:1009-16 (2015).

Cites ab104224

Aoki S  et al. Role of Striatal Cholinergic Interneurons in Set-Shifting in the Rat. J Neurosci 35:9424-31 (2015).

Cites ab104224

Sun J  et al. Electroacupuncture alleviates retrieval of pain memory and its effect on phosphorylation of cAMP response element-binding protein in anterior cingulate cortex in rats. Behav Brain Funct 11:9 (2015).

Cites ab104224

Masson GS  et al. Toll-like receptor 4 promotes autonomic dysfunction, inflammation and microglia activation in the hypothalamic paraventricular nucleus: role of endoplasmic reticulum stress. PLoS One 10:e0122850 (2015).

Cites ab104224

Yu P  et al. Characterization of brain cell nuclei with decondensed chromatin. Dev Neurobiol N/A:N/A (2014).

Cites ab104224

Li XQ  et al. Sevoflurane preconditioning ameliorates neuronal deficits by inhibiting microglial MMP-9 expression after spinal cord ischemia/reperfusion in rats. Mol Brain 7:69 (2014).

Cites ab104224

Farrington GK  et al. A novel platform for engineering blood-brain barrier-crossing bispecific biologics. FASEB J 28:4764-78 (2014).

Cites ab104224

Kaur P  et al. Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS One 9:e103525 (2014).

Cites ab104224

Patel TP  et al. Single-neuron NMDA receptor phenotype influences neuronal rewiring and reintegration following traumatic injury. J Neurosci 34:4200-13 (2014).

Cites ab104224

Derlig K  et al. Identification and characterisation of Simiate, a novel protein linked to the fragile X syndrome. PLoS One 8:e83007 (2013).

Cites ab104224

Chio CC  et al. Etanercept attenuates traumatic brain injury in rats by reducing early microglial expression of tumor necrosis factor-a. BMC Neurosci 14:33 (2013).

Cites ab104224

Huang JF  et al. Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response. BMC Neurosci 14:16 (2013).

Hodgkin J, Zellan JD, Albertson DG. Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans. Development 120:3681-3689 (1994).

Kim KK, Adelstein RS, Kawamoto S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284:31052-31061 (2009).

Underwood,J.G., Boutz,P.L., Dougherty,J.D., Stoilov,P. and Black,D.L. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol. Cell. Biol. 25:10005-10016 (2005).

Herculano-Houzel S, Lent R. Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain. J Neurosci. 25:2518-2521 (2005).

Mullen RJ, Buck CR, Smith AM. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116:201-211 (1994).

Rating

Species

Application

Date

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Mouse

IHC-P

16-May-2016

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Marmoset

IHC-P

01-Apr-2016

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Chicken

IHC-P

01-Apr-2016

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Rat

IHC-P

01-Apr-2016

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Sea Urchin

IHC-P

01-Apr-2016

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Porcine

IHC-P

01-Apr-2016

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Canine

IHC-P

01-Apr-2016

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Dog

IHC-P

01-Apr-2016

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Goat

IHC-P

01-Apr-2016


start icons start icons start icons start icons start icons (10)
Total Citations (37)
Description: Rabbit polyclonal antibody to Fox3.
Application: WB, ICC/IF, IHC
Host: Rabbit
Isotype: IgG
Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse

£165 – £714

Description: Rabbit polyclonal antibody to RBFOX3.
Application: WB, IHC, IF
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£148 – £372

Total Citations (3)
Description: Mouse monoclonal (1B7) antibody to Fox3.
Application: WB, ICC/IF, IHC
Host: Mouse
Isotype: IgG2a
Immunogen: N-terminal 100 amino acids of human Fox3 expressed in and purified from E. coli.

£165 – £714

Description: Rabbit monoclonal antibody to NeuN
Application: WB, ICC/IF, IHC, FC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant antibody.

£269 – £400

Please Note: Anti-Fox3/NeuN Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | MCA-1B7-AP
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100µl £230
500µl £714

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