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Anti-GAPDH Antibody

 
Total Citations (9)
Mouse monoclonal (1D4) antibody to GAPDH from EnCor Biotechnology (MCA-1D4-AP).
Name: Anti-GAPDH Antibody
Description: Mouse monoclonal (1D4) antibody to GAPDH.
Applications: WB, ICC/IF, IHC
Dilutions: WB: 1:1,000. IF/IHC: 1:100.
Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse
Immunogen: Whole protein purified from pig.
Host: Mouse
Clonality: Monoclonal
Clone: 1D4
Isotype: IgM
Conjugate: Unconjugated
Purification: Immunogen affinity purified.
Concentration: 1 mg/ml
Molecular Weight: 38 kDa
Product Form: Liquid
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Introduction: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein is expressed in large amounts and which is required at all times for an important “house keeping” functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles, such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer’s disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington’s disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). The immunogen used to raise this particular antibody was extensively purified pig GAPDH. The HGNC name for this protein is GAPDH.
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Immunohistochemisty - Anti-MCA-1D4 Antibody from EnCor Biotechnology (MCA-1D4-AP) - Antibodies.com
Human neuroblastoma SH-SY5Y cells stained with MCA-1D4-AP (green), chicken antibody to neurofilament NF-H, CPCA-NF-H, (red) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.
Western Blot - Anti-MCA-1D4 Antibody from EnCor Biotechnology (MCA-1D4-AP) - Antibodies.com
Blots of mice peripheral nerves blotted with MCA-1D4-AP for use as an western blotting control. Note the single sharp band corresponding to GAPDH.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies.

Inducible HSP70 is critical in preventing the aggregation and enhancing the processing of PMP22.

Biochemical characterization of protein quality control mechanisms during disease progression in the C22 mouse model of CMT1A.

Regulation of dipeptidyl peptidase 8 and 9 expression in activated lymphocytes and injured liver.

Influence of late-life exposure to environmental enrichment or exercise on hippocampal function and CA1 senescent physiology.

A novel role of dipeptidyl peptidase 9 in epidermal growth factor signaling.

Lifelong calorie restriction alleviates age-related oxidative damage in peripheral nerves.

Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging.

The heme precursor delta-aminolevulinate blocks peripheral myelin formation.

Burke JR, Enghild JJ, Martin ME, Jou Y-S, Myers RM, Roses AD, Vance JM & Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature Med. 2: 347-350, 1996. 

Schulze H, Schuler A, Stuber D, Dobeli H, Langern H & Huber G. Rat brain glyceraldehyde-3-phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer’s beta-amyloid precursor protein. J Neurochem. 60:1915-22 1993

Morgenegg G, Winkler GC, Hubscher U, Heizmann CW, Mous J, Kuenzle CC. Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. J Neurochem. 47:54-62 1986

Lee WH, Kumar A, Rani A, Herrera J, Xu J, Someya S, Foster TC. Influence of viral vector-mediated delivery of superoxide dismutase and catalase to the hippocampus on spatial learning and memory during aging. Antioxid Redox Signal. 16:339-50 2012

Kumar A, Rani A, Tchigranova O, Lee WH, Foster TC. Influence of late-life exposure to environmental enrichment or exercise on hippocampal function and CA1 senescent physiology. Neurobiol Aging. 2011 Aug 3. [Epub ahead of print]

Verrier JD, Madorsky I, Coggin WE, Geesey M, Hochman M, Walling E, Daroszewski D, Eccles KS, Ludlow R, Semple-Rowland SL. Bicistronic lentiviruses containing a viral 2A cleavage sequence reliably co-express two proteins and restore vision to an animal model of LCA1. PLoS One. 6:e20553 2011

Zeier Z, Madorsky I, Xu Y, Ogle WO, Notterpek L, Foster TC. Gene Expression in the Hippocampus: Regionally Specific Effects of Aging and Caloric Restriction. Mech Ageing Dev. 132:8-19 2011

Verrier JD, Semple-Rowland S, Madorsky I, Papin JE, Notterpek L. Reduction of Dicer impairs Schwann cell differentiation and myelination. J. Neurosci Res. 88:2558-68 2010.

Opalach K, Rangaraju S, Madorsky I, Leeuwenburgh C, Notterpek L. Lifelong calorie restriction alleviates age-related oxidative damage in peripheral nerves. Rejuvenation Res. 13:65-74 2010.

Madorsky I, Opalach K, Waber A, Verrier JD, Solmo C, Foster T, Dunn WA Jr, Notterpek L. Intermittent fasting alleviates the neuropathic phenotype in a mouse model of Charcot-Marie-Tooth disease. Neurobiol Dis. 34:146-54 2009

Rangaraju S, Hankins D, Madorsky I, Madorsky E, Lee WH, Carter CS, Leeuwenburgh C, Notterpek L.Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 8:178-91 2009

Verrier JD, Lau P, Hudson L, Murashov AK, Renne R, Notterpek L. Peripheral myelin protein 22 is regulated post-transcriptionally by miRNA-29a. Glia 57:1265-79 2009

Lau P, Verrier JD, Nielsen JA, Johnson KR, Notterpek L, Hudson LD. Identification of dynamically regulated microRNA and mRNA networks in developing oligodendrocytes. J Neurosci. 28:11720-30 2008

Felitsyn N, McLeod C, Shroads AL, Stacpoole PW, Notterpek L. The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 106:2068-79 2008

Rangaraju S, Madorsky I, Pileggi JG, Kamal A, Notterpek L. Pharmacological induction of the heat shock response improves myelination in a neuropathic model. Neurobiol Dis. 32:105-15 2008

Felitsyn N, Stacpoole, PW & Nottepek L. Dichloroacetate causes reversible demyelination in vitro: potential mechanism for its neuropathic effect. J. Neurochem 100:429-36 2007

Amici SA, Dunn WA & Notterpek, L. Developmental abnormalities in the nerves of peripheral myelin protein 22-deficient mice. J. Neurosci. Res. 85:238-249 2006

Amici SA, Dunn WA, Murphy AJ, Adams NC, Gale NW, Valenzuela DM, Yancopoulos GD & Notterpek L.Peripheral Myelin Protein 22 Is in Complex with {alpha}6beta4 Integrin, and Its Absence Alters the Schwann Cell Basal Lamina. J. Neurosci. 26:1179-89 2006

Fortun J, Go JC, Li J, Amici SA, Dunn WA Jr, Notterpek L. Alterations in degradative pathways and protein aggregation in a neuropathy model based on PMP22 overexpression. Neurobiol Dis. 22:153-164 2006

Iskandar M, Swist E, Trick KD, Wang B, L’Abbe MR, Bertinato J. Copper chaperone for Cu/Zn superoxide dismutase is a sensitive biomarker of mild copper deficiency induced by moderately high intakes of zinc. Nutr J. 4:35 2005

Fortun J, Li J, Go J, Fenstermaker A, Fletcher BS & Notterpek L. Impaired proteasome activity and accumulation of ubiquitinated substrates in a hereditary neuropathy model. J Neurochem. 92:1531-41 2005

Ellis RC, Earnhardt JN, Hayes RL, Wang KK & Anderson DK. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J Neurochem. 88:689-97 2004.

Fortun J, Dunn WA, Joy S, Li J. & Notterpek, L. Emerging Role for Autophagy in the Removal of Aggresomes in Schwann Cells. J. Neurosci. 23:10672-10680 2003.

Dastoor Z. & Dreyer, J-L. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. J. Cell Sci. 114:1643-1653 2001.

Total Citations (117)
Description: Rabbit polyclonal antibody to GAPDH
Application: WB
Host: Rabbit
Reactivity: Human, Mouse, Rat Mammals
Immunogen: Recombinant full length Human GAPDH.

£157 – £269

Total Citations (104)
Description: Mouse monoclonal antibody to GAPDH (1A6)
Application: ELISA, WB, IHC, IF
Host: Mouse
Reactivity: Human, Mouse, Rabbit, Frog, Fish, Chicken, Rat
Immunogen: Recombinant full length Human GAPDH.

£157 – £269

start icons start icons start icons start icons start icons (2)
Total Citations (34)
Description: Goat polyclonal antibody to GAPDH.
Application: ELISA, WB, IF, IHC
Host: Goat
Isotype: IgG
Reactivity: Human, Mouse, Rat

£205

Total Citations (24)
Description: Rabbit polyclonal antibody to GAPDH (A531)
Application: WB
Host: Rabbit
Reactivity: Human, Mouse, Rat Mammals
Immunogen: Recombinant full length Human GAPDH.

£157 – £269

Please Note: Anti-GAPDH Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | MCA-1D4-AP
Size Price Quantity
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100µl £230
500µl £714

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