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Anti-GAPDH Antibody

Total Citations (117)
Rabbit polyclonal antibody to GAPDH from Bioworld Technology (AP0063).
Name: Anti-GAPDH Antibody
Description: Rabbit polyclonal antibody to GAPDH
Specificity: GAPDH pAb detects endogenous levels of GAPDH protein.
Applications: WB
Reactivity: Human, Mouse, Rat Mammals
Immunogen: Recombinant full length Human GAPDH.
Host: Rabbit
Clonality: Polyclonal
Conjugate: Unconjugated
Molecular Weight: ~ 36 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Product Form: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Introduction: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
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Anti-GAPDH Antibody from Bioworld Technology (AP0063) - Antibodies.com
For details please refer to datasheet.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

A novel nickel complex works as a proteasomal deubiquitinase inhibitor for cancer therapy. Oncogene (2016) Apr 18 :

Cdc42 deficiency induces podocyte apoptosis by inhibiting the Nwasp/stress fibers/YAP pathway. Cell Death Dis (2016) 7 : e2142

MiR-582-5p/miR-590-5p targeted CREB1/CREB5-NF-B signaling and caused opioid-induced immunosuppression in human monocytes. Transl Psychiatry (2016) 6 : e757

miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6. Oncol Lett (2016) Jan 11 1: 543-550

Inhibition of Mitochondrial Fission and NOX2 Expression Prevent NLRP3 Inflammasome Activation in the Endothelium: The Role of Corosolic Acid Action in the Amelioration of Endothelial Dysfunction. Antioxid. Redox Signal. (2016) Mar 22 :

PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells. Sci Rep (2016) 6 : 20823

Corilagin Attenuates Radiation-Induced Brain Injury in Mice. Mol. Neurobiol. (2015) Dec 15 :

Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation. Mol Nutr Food Res (2016) Mar 60 3: 551-65

Oncogenic miR-9 is a target of erlotinib in NSCLCs. Sci Rep (2015) 5 : 17031

An integrated pathway interaction network for the combination of four effective compounds from ShengMai preparations in the treatment of cardio-cerebral ischemic diseases. Acta Pharmacol. Sin. (2015) Nov 36 11: 1337-48

Caruifolin D from artemisia absinthium L. inhibits neuroinflammation via reactive oxygen species-dependent c-jun N-terminal kinase and protein kinase c/NF-B signaling pathways. Eur. J. Pharmacol. (2015) Nov 15 767 : 82-93

Interferon- and tumor necrosis factor- promote the ability of human placenta-derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4(+)interleukin-10(+) and CD8(+)interleukin-10(+)Treg subsets. Cytotherapy (2015) Nov 17 11: 1560-71

Curcumin alleviated the toxic reaction of Rhizoma Paridis saponins in a 45-day subchronic toxicological assessment of rats. Environ. Toxicol. (2015) Sep 22 :

GL-V9, a new synthetic flavonoid derivative, ameliorates DSS-induced colitis against oxidative stress by up-regulating Trx-1 expression via activation of AMPK/FOXO3a pathway. Oncotarget (2015) Sep 22 6 28: 26291-307

8-Methoxypsoralen Induces Intrinsic Apoptosis in HepG2 Cells: Involvement of Reactive Oxygen Species Generation and ERK1/2 Pathway Inhibition. Cell. Physiol. Biochem. (2015) 37 1: 361-74

Diosgenin regulates adipokine expression in perivascular adipose tissue and ameliorates endothelial dysfunction via regulation of AMPK. J. Steroid Biochem. Mol. Biol. (2016) Jan 155 Pt A: 155-65

Non-classical MHC I-E negatively regulates macrophage activation and Th17 cell development in NOD mice. Sci Rep (2015) 5 : 12941

iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG. Front Microbiol (2015) 6 : 691

Pharmacological activation of AMPK prevents Drp1-mediated mitochondrial fission and alleviates endoplasmic reticulum stress-associated endothelial dysfunction. J. Mol. Cell. Cardiol. (2015) Sep 86 : 62-74

Endoplasmic reticulum ribosome-binding protein 1, RRBP1, promotes progression of colorectal cancer and predicts an unfavourable prognosis. Br. J. Cancer (2015) Sep 1 113 5: 763-72

Iptakalim inhibits PDGF-BB-induced human airway smooth muscle cells proliferation and migration. Exp. Cell Res. (2015) Aug 15 336 2: 204-10

Hydrogen Sulfide Attenuates Inflammatory Hepcidin by Reducing IL-6 Secretion and Promoting SIRT1-Mediated STAT3 Deacetylation. Antioxid. Redox Signal. (2016) Jan 10 24 2: 70-83

The oncogene c-Jun impedes somatic cell reprogramming. Nat. Cell Biol. (2015) Jul 17 7: 856-67

Neuroprotection of Sanhua Decoction against Focal Cerebral Ischemia/Reperfusion Injury in Rats through a Mechanism Targeting Aquaporin 4. Evid Based Complement Alternat Med (2015) 2015 : 584245

Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction. Stem Cells Int (2015) 2015 : 176409

Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner. Pharmacol. Res. (2015) Sep 99 : 101-15

The influence of maternal dietary fat on the fatty acid composition and lipid metabolism in the subcutaneous fat of progeny pigs. Meat Sci. (2015) Oct 108 : 82-7

MicroRNA-218 inhibits the proliferation and metastasis of esophageal squamous cell carcinoma cells by targeting BMI1. Int. J. Mol. Med. (2015) Jul 36 1: 93-102

Respiratory Syncytial Virus Infection Upregulates NLRC5 and Major Histocompatibility Complex Class I Expression through RIG-I Induction in Airway Epithelial Cells. J. Virol. (2015) Aug 89 15: 7636-45

Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium. J Ethnopharmacol (2015) Jul 1 169 : 210-8

Reg3 Overexpression Protects Pancreatic Beta-Cells From Cytokine-Induced Damage and Improves Islet Transplant Outcome. Mol. Med. (2014) Dec 17 :

The PI3K/Akt signaling pathway mediates the high glucose-induced expression of extracellular matrix molecules in human retinal pigment epithelial cells. J Diabetes Res (2015) 2015 : 920280

Akt1 signaling coordinates BMP signaling and -catenin activity to regulate second heart field progenitor development. Development (2015) Feb 15 142 4: 732-42

Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model. PLoS ONE (2015) 10 2: e0117432

Differential drug resistance acquisition to doxorubicin and paclitaxel in breast cancer cells. Cancer Cell Int. (2014) 14 1: 538

SC-III3, a novel scopoletin derivative, induces cytotoxicity in hepatocellular cancer cells through oxidative DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation. BMC Cancer (2014) 14 : 987

Alternation of adriamycin penetration kinetics in MCF-7 cells from 2D to 3D culture based on P-gp expression through the Chk2/p53/NF-B pathway. Biochem. Pharmacol. (2015) Jan 15 93 2: 210-20

Fungal metabolite myriocin promotes human herpes simplex virus-2 infection. Life Sci. (2015) Jan 1 120 : 31-8

Rapamycin protects against apoptotic neuronal death and improves neurologic function after traumatic brain injury in mice via modulation of the mTOR-p53-Bax axis. J. Surg. Res. (2015) Mar 194 1: 239-47

Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats. Acta Physiol (Oxf) (2014) Dec 212 4: 267-82

Overexpression of Chemokine (C-X-C) ligand 1 (CXCL1) associated with tumor progression and poor prognosis in hepatocellular carcinoma. Cancer Cell Int. (2014) 14 1: 86

Identification and characterization of alphavirus M1 as a selective oncolytic virus targeting ZAP-defective human cancers. Proc. Natl. Acad. Sci. U.S.A. (2014) Oct 21 111 42: E4504-12

Alpha-lipoic acid upregulates SIRT1-dependent PGC-1 expression and protects mouse brain against focal ischemia. Neuroscience (2014) Oct 2 281C : 251-257

Effects of gastrokine2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways. Mol Med Rep (2014) Dec 10 6: 2898-904

Regional arterial infusion with lipoxin A4 attenuates experimental severe acute pancreatitis. PLoS ONE (2014) 9 9: e108525

MTA1 regulates higher-order chromatin structure and histone H1-chromatin interaction in-vivo. Mol Oncol (2015) Jan 9 1: 218-35

A selenosemicarbazone complex with copper efficiently down-regulates the 90-kDa heat shock protein HSP90AA1 and its client proteins in cancer cells. BMC Cancer (2014) 14 : 629

Anti-metastasis effect of fucoidan from Undaria pinnatifida sporophylls in mouse hepatocarcinoma Hca-F cells. PLoS ONE (2014) 9 8: e106071

The transcription factor FOXA2 suppresses gastric tumorigenesis in vitro and in vivo. Dig. Dis. Sci. (2015) Jan 60 1: 109-17

Pharmacological activation of AMPK ameliorates perivascular adipose/endothelial dysfunction in a manner interdependent on AMPK and SIRT1. Pharmacol. Res. (2014) Nov 89 : 19-28

MicroRNA-mediated repression of nonsense mRNAs. Elife (2014) 3 : e03032

Fluoxetine suppresses AMP-activated protein kinase signaling pathway to promote hepatic lipid accumulation in primary mouse hepatocytes. Int. J. Biochem. Cell Biol. (2014) Sep 54 : 236-44

CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression. J. Cancer Res. Clin. Oncol. (2015) Jan 141 1: 21-Nov

Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Oncotarget (2014) Aug 15 5 15: 6365-74

LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/-catenin signaling pathway in colorectal cancer cells. Cell. Signal. (2014) Nov 26 11: 2333-42

MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c-MET. Oncol Lett (2014) Aug 8 2: 891-897

MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma. Oncotarget (2014) Jul 30 5 14: 5416-27

Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth. Oncotarget (2014) Jul 30 5 14: 5453-71

ID2 predicts poor prognosis in breast cancer, especially in triple-negative breast cancer, and inhibits E-cadherin expression. Onco Targets Ther (2014) 7 : 1083-94

The subcellular distribution and function of MTA1 in cancer differentiation. Oncotarget (2014) Jul 15 5 13: 5153-64

Cardioprotective effect of betulinic Acid on myocardial ischemia reperfusion injury in rats. Evid Based Complement Alternat Med (2014) 2014 : 573745

A novel proteasome inhibitor suppresses tumor growth via targeting both 19S proteasome deubiquitinases and 20S proteolytic peptidases. Sci Rep (2014) 4 : 5240

IQGAP3 promotes EGFR-ERK signaling and the growth and metastasis of lung cancer cells. PLoS ONE (2014) 9 5: e97578

Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation. J. Immunol. (2014) May 15 192 10: 4758-64

Cytoplasmic retention of a nucleocytoplasmic protein TBC1D3 by microtubule network is required for enhanced EGFR signaling. PLoS ONE (2014) 9 4: e94134

Bursopentin (BP5) from chicken bursa of fabricius attenuates the immune function of dendritic cells. Amino Acids (2014) Jul 46 7: 1763-74

mTOR-targeted therapy: differential perturbation to mitochondrial membrane potential and permeability transition pore plays a role in therapeutic response. Biochem. Biophys. Res. Commun. (2014) Apr 25 447 1: 184-91

Phosphorylation of heat shock protein 27 antagonizes TNF- induced HeLa cell apoptosis via regulating TAK1 ubiquitination and activation of p38 and ERK signaling. Cell. Signal. (2014) Jul 26 7: 1616-25

Lack of seipin in neurons results in anxiety- and depression-like behaviors via down regulation of PPAR. Hum. Mol. Genet. (2014) Aug 1 23 15: 4094-102

Catalpol promotes oligodendrocyte survival and oligodendrocyte progenitor differentiation via the Akt signaling pathway in rats with chronic cerebral hypoperfusion. Brain Res. (2014) Apr 29 1560 : 27-35

Asiaticoside attenuates memory impairment induced by transient cerebral ischemia-reperfusion in mice through anti-inflammatory mechanism. Pharmacol. Biochem. Behav. (2014) Jul 122 : 15-Jul

Overexpression of BAT3 alleviates prion protein fragment PrP106-126-induced neuronal apoptosis. CNS Neurosci Ther (2014) Aug 20 8: 737-47

Cytohesins/ARNO: the function in colorectal cancer cells. PLoS ONE (2014) 9 3: e90997

Resveratrol prevents neuronal apoptosis in an early brain injury model. J. Surg. Res. (2014) Jun 1 189 1: 159-65

Sophocarpine attenuates liver fibrosis by inhibiting the TLR4 signaling pathway in rats. World J. Gastroenterol. (2014) Feb 21 20 7: 1822-32

The Epstein-Barr virus-encoded microRNA MiR-BART9 promotes tumor metastasis by targeting E-cadherin in nasopharyngeal carcinoma. PLoS Pathog. (2014) Feb 10 2: e1003974

The LEF1/CYLD axis and cIAPs regulate RIP1 deubiquitination and trigger apoptosis in selenite-treated colorectal cancer cells. Cell Death Dis (2014) 5 : e1085

miR-203 inhibits tumor cell migration and invasion via caveolin-1 in pancreatic cancer cells. Oncol Lett (2014) Mar 7 3: 658-662

Snail contributes to the maintenance of stem cell-like phenotype cells in human pancreatic cancer. PLoS ONE (2014) 9 1: e87409

SIRT1 inhibition by sirtinol aggravates brain edema after experimental subarachnoid hemorrhage. J. Neurosci. Res. (2014) Jun 92 6: 714-22

An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation. Mol. Biol. Rep. (2014) May 41 5: 2757-65

Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells. PLoS ONE (2014) 9 1: e87223

Pregnancy-induced physiological hypertrophy protects against cardiac ischemia-reperfusion injury. Int J Clin Exp Pathol (2014) 7 1: 229-35

Cardiac ablation of Rheb1 induces impaired heart growth, endoplasmic reticulum-associated apoptosis and heart failure in infant mice. Int J Mol Sci (2013) 14 12: 24380-98

Microvesicle-shuttled miR-130b reduces fat deposition in recipient primary cultured porcine adipocytes by inhibiting PPAR-g expression. J. Cell. Physiol. (2014) May 229 5: 631-9

Extracellular polysaccharide from Bacillus sp. strain LBP32 prevents LPS-induced inflammation in RAW 264.7 macrophages by inhibiting NF-B and MAPKs activation and ROS production. Int. Immunopharmacol. (2014) Jan 18 1: 12-9

Indole conjugated silica and magnetic nanoparticles as inhibitors of HIF. Colloids Surf B Biointerfaces (2014) Feb 1 114 : 158-63

Contrasting effects of Krppel-like factor 4 on X-ray-induced double-strand and single-strand DNA breaks in mouse astrocytes. Cell Biochem. Funct. (2014) Apr 32 3: 241-8

Expression of Rac-1 related to tumor depth, lymph node metastasis and patient prognosis in esophageal squamous cell carcinoma. Med. Oncol. (2013) Dec 30 4: 689

NBPF is a potential DNA-binding transcription factor that is directly regulated by NF-B. Int. J. Biochem. Cell Biol. (2013) Nov 45 11: 2479-90

Inhibition of SIRT1 combined with gemcitabine therapy for pancreatic carcinoma. Clin Interv Aging (2013) 8 : 889-97

Autophagy contributes to the survival of CD133+ liver cancer stem cells in the hypoxic and nutrient-deprived tumor microenvironment. Cancer Lett. (2013) Oct 1 339 1: 70-81

Promotion of melanoma cell invasion and tumor metastasis by microcystin-LR via phosphatidylinositol 3-kinase/AKT pathway. Environ. Sci. Technol. (2013) Aug 6 47 15: 8801-8

Opposite effects of genistein on the regulation of insulin-mediated glucose homeostasis in adipose tissue. Br. J. Pharmacol. (2013) Sep 170 2: 328-40

miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6. FEBS Lett. (2013) Jun 19 587 12: 1754-61

Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth. Oncol. Rep. (2013) Jun 29 6: 2422-30

Impact of high salt independent of blood pressure on PRMT/ADMA/DDAH pathway in the aorta of Dahl salt-sensitive rats. Int J Mol Sci (2013) 14 4: 8062-72

Anti-ABCG2 monoclonal antibody in combination with paclitaxel nanoparticles against cancer stem-like cell activity in multiple myeloma. Nanomedicine (Lond) (2014) Jan 9 1: 45-60

Unexpected Nephrotoxicity in Male Ablactated Rats Induced by Cordyceps militaris: The Involvement of Oxidative Changes. Evid Based Complement Alternat Med (2013) 2013 : 786528

Vinexin- protects against cardiac hypertrophy by blocking the Akt-dependent signalling pathway. Basic Res. Cardiol. (2013) Mar 108 2: 338

miR-154 inhibits prostate cancer cell proliferation by targeting CCND2. Urol. Oncol. (2014) Jan 32 1: 31.e9-16

hSSB1 regulates both the stability and the transcriptional activity of p53. Cell Res. (2013) Mar 23 3: 423-35

Human umbilical cord stem cells ameliorate experimental autoimmune encephalomyelitis by regulating immunoinflammation and remyelination. Stem Cells Dev. (2013) Apr 1 22 7: 1053-62

Adrenomedullin attenuates vascular calcification in fructose-induced insulin resistance rats. Acta Physiol (Oxf) (2013) Mar 207 3: 437-46

Phycocyanin may suppress D-galactose-induced human lens epithelial cell apoptosis through mitochondrial and unfolded protein response pathways. Toxicol. Lett. (2012) Nov 23 215 1: 25-30

JNK pathway decreases thyroid hormones via TRH receptor: a novel mechanism for disturbance of thyroid hormone homeostasis by PCB153. Toxicology (2012) Dec 8 302 1: 68-76

Perifosine enhances mTORC1-targeted cancer therapy by activation of GSK3 in NSCLC cells. Cancer Biol. Ther. (2012) Sep 13 11: 1009-17

Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through Fas- and mitochondria-mediated pathways. Mol. Carcinog. (2013) Dec 52 12: 983-96

Down regulation of differentiated embryonic chondrocytes 1 (DEC1) is involved in 8-methoxypsoralen-induced apoptosis in HepG2 cells. Toxicology (2012) Nov 15 301 1-3: 58-65

Natural products as a gold mine for selective matrix metalloproteinases inhibitors. Bioorg. Med. Chem. (2012) Jul 1 20 13: 4164-71

miR-181a/b significantly enhances drug sensitivity in chronic lymphocytic leukemia cells via targeting multiple anti-apoptosis genes. Carcinogenesis (2012) Jul 33 7: 1294-301

Opsin3 sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by regulating the apoptotic pathway. Cancer Lett. (2012) Jul 1 320 1: 96-103

Omega-3-polyunsaturated fatty acids suppress pancreatic cancer cell growth in vitro and in vivo via downregulation of Wnt/Beta-catenin signaling. Pancreatology (2011) 11 6: 574-84

The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR. Proteomics (2012) Jan 12 2: 300-12

miR-200bc/429 cluster modulates multidrug resistance of human cancer cell lines by targeting BCL2 and XIAP. Cancer Chemother. Pharmacol. (2012) Mar 69 3: 723-31

miR-106a-mediated malignant transformation of cells induced by anti-benzo[a]pyrene-trans-7,8-diol-9,10-epoxide. Toxicol. Sci. (2011) Jan 119 1: 50-60

Human urinary kallidinogenase suppresses cerebral inflammation in experimental stroke and downregulates nuclear factor-kappaB. J. Cereb. Blood Flow Metab. (2010) Jul 30 7: 1356-65

Total Citations (104)
Description: Mouse monoclonal antibody to GAPDH (1A6)
Application: ELISA, WB, IHC, IF
Host: Mouse
Reactivity: Human, Mouse, Rabbit, Frog, Fish, Chicken, Rat
Immunogen: Recombinant full length Human GAPDH.

£157 – £269

start icons start icons start icons start icons start icons (2)
Total Citations (34)
Description: Goat polyclonal antibody to GAPDH.
Application: ELISA, WB, IF, IHC
Host: Goat
Isotype: IgG
Reactivity: Human, Mouse, Rat


Total Citations (24)
Description: Rabbit polyclonal antibody to GAPDH (A531)
Application: WB
Host: Rabbit
Reactivity: Human, Mouse, Rat Mammals
Immunogen: Recombinant full length Human GAPDH.

£157 – £269

Total Citations (9)
Description: Mouse monoclonal (1D4) antibody to GAPDH.
Application: WB, ICC/IF, IHC
Host: Mouse
Isotype: IgM
Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse

£165 – £714

Please Note: Anti-GAPDH Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | AP0063
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100µl £269

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