+44 (0)1223 298875

Anti-GFAP Antibody

Rabbit polyclonal antibody to GFAP from EnCor Biotechnology (RPCA-GFAP).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab7260
Name: Anti-GFAP Antibody
Description: Rabbit polyclonal antibody to GFAP.
Applications: WB, ICC/IF, IHC
Dilutions: WB: 1:5,000. IF/ICC and IHC 1:1,000-1,5,000.
Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse
Immunogen: Recombinant human full length GFAP protein.
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Conjugate: Unconjugated
Molecular Weight: 50 kDa
Purity: Whole antiserum.
Product Form: Liquid
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Introduction: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and coworkers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states, GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander’s disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (2). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes. The HGNC name for this protein is GFAP. The initial challenge was performed with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with GFAP purified from a Triton X-100 extract of myelin associated material from bovine spinal cord, following an “axonal flotation” procedure (3). The GFAP was further purified by centrifugation and ion exchange chromatography in 6M urea on DEAE cellulose. This antibody is provided as crude serum and has an extremely high titer (see recommended use).
Synonyms: wu:fb34h11 Antibody
ALXDRD Antibody
cb345 Antibody
etID36982.3 Antibody
FLJ42474 Antibody
FLJ45472 Antibody
gfapl Antibody
GFAP_HUMAN Antibody
Glial fibrillary acidic protein Antibody
Intermediate filament protein Antibody
wu:fk42c12 Antibody
xx:af506734 Antibody
zgc:110485 Antibody
Immunohistochemisty - Anti-GFAP Antibody from EnCor Biotechnology (RPCA-GFAP) - Antibodies.com
Mixed neuron-glial cultures stained with rabbit GFAP (red channel) and chicken vimentin CPCA-Vim (green channel). The fibroblastic cells contain only vimentin and so are green, while astrocytes contain either vimentin and GFAP, so appearing golden, or predominantly GFAP, in which case they appear red. Blue is nuclear DNA stain.
Western Blot - Anti-GFAP Antibody from EnCor Biotechnology (RPCA-GFAP) - Antibodies.com
Western blot analysis of RPCA-GFAP.  Blot of rat brain lysate was probed with RPCA-GFAP, at dilution of 1:5,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

Liem RKH, Yen SH, Salomon GD and Shelanski ML. Intermediate filaments in nervous tissues. J Cell Biol 79:637-745 (1978).

Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 1972.

Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 2001

Description: Goat polyclonal antibody to GFAP.
Application: ELISA, WB
Host: Goat
Isotype: IgG
Reactivity: Human, Mouse, Rat

£205

Description: Rabbit polyclonal antibody to GFAP
Application: WB, IHC, IF
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human GFAP .

£234

Description: Rabbit monoclonal antibody to GFAP.
Application: WB, IHC, IP
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£372 – £565

Description: Mouse monoclonal antibody to GFAP
Application: WB, IHCICCFC
Host: Mouse
Reactivity: Human, Mouse, Rat
Immunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of GFAP.

£269 – £400

Please Note: Anti-GFAP Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | RPCA-GFAP
Size Price Quantity
50µl £165
100µl £230
500µl £714

or
Request a quotation
Contact Us Directly
  • call icon + 44 (0)1223 298 875
  • + 44 (0)1223 281 447
  • email icon info@antibodies.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Lead Time: Currently 3-4 Days
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe