+44 (0)1223 298875

Anti-Histone H2AX Antibody

Mouse monoclonal antibody to Histone H2AX from ABclonal (A0823).
Name: Anti-Histone H2AX Antibody
Description: Mouse monoclonal antibody to Histone H2AX.
Applications: WB, IHC
Dilutions: WB: 1:500 - 1:1000, IHC: 1:50 - 1:100.
Reactivity: Human
Immunogen: A synthetic peptide of human H2AFX.
Protein Length: 143
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Sequence Similarities: Belongs to the histone H2A family.
Post-Translational Modification: Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).
Cellular Location: Nucleus. Chromosome.
UniProt: P16104
Gene ID: 3014
Synonyms: AW228881 Antibody
H2A histone family member X Antibody
H2A.FX Antibody
H2A.X Antibody
H2a/x Antibody
H2AFX Antibody
H2AX Antibody
H2AX histone Antibody
H2AX_HUMAN Antibody
Hist5.2ax Antibody
Histone 2A Antibody
Histone 2AX Antibody
Histone H2A.X Antibody
RGD1566119 Antibody
Information: Target information shown above is from the UniProt Consortium.
Western blot analysis of extracts of Raji cells tissue, using H2AFX antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.
Description: Rabbit polyclonal antibody to Histone H2AX
Application: WB, IF
Host: Rabbit
Reactivity: Human
Immunogen: Synthesized peptide derived from internal of human Histone H2AX.

£180 – £234

Description: Rabbit polyclonal antibody to Histone H2AX.
Application: WB, IHC, ICC
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£243 – £565

Description: Rabbit polyclonal antibody to Histone H2AX.
Application: WB, IHC, IF, IP
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£148 – £372

Description: Rabbit polyclonal antibody to Histone H2AX.
Application: WB, IF
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse

£256





Please Note: Anti-Histone H2AX Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A0823
Size Price Quantity
100µl £243
200µl £372

or
Request a quotation
Contact Us Directly
  • call icon + 44 (0)1223 298 875
  • + 44 (0)1223 281 447
  • email icon info@antibodies.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Lead Time: Currently 4-6 Days
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe