Mouse monoclonal [PCRP-AGO3-1C5] antibody to AGO3.
Specificità
This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as argonaute RISC catalytic component 3.Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
SDS-PAGE analysis of Anti-AGO3 Antibody [PCRP-AGO3-1C5] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Flow cytometric analysis of PFA-fixed HeLa cells using Anti-AGO3 Antibody [PCRP-AGO3-1C5] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-AGO3 Antibody [PCRP-AGO3-1C5] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-AGO3 Antibody [PCRP-AGO3-1C5]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.