Figure 1: Western Blot - Anti-Akt1 (phospho Ser473) Antibody [X20-A] (A8277)
(A) Western blot analysis of Akt activation in striatal neurons stimulated with DHPG (mGluR5 agonist) for 0 (Lane 1), 2 (Lane 2), 5 (Lane 3) or 10 (Lane 4) minutes. (B) Western blot analysis of total Akt, detected with Anti-Akt1 (phospho Ser473) Antibody (A8277) in striatal neurons stimulated with DHPG (a mGluR5 agonist) for 0 (Lane 1), 2 (Lane 2), 5 (Lane 3) or 10 (Lane 4) minutes. Wells were equally loaded with 100µg of whole cell lysate proteins.
Representative picture of Akt1 (only when phosphorylated at the Ser-473 residue) in HEK293 cells, visualized with Anti-Akt1 (phospho Ser473) Antibody (A8277). Primary antibody dilution: 1:300.
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