Figure 1: Western Blot - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568)
Western blot analysis of extracts of HeLa cells, using Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Prodotti alternativi a Anti-alpha 1 Fetoprotein Anticorpo [AMC0201] (A308568)