Recombinant construct corresponding to the C-terminal 398 amino acids of human Ankyrin 3 isotype 1 (NP_066267.2), expressed in and purified from E. coli. This segment is expressed by all three Ankyrin 3 isotypes and contains the DEATH domain sequence.
Specie ospite
Chicken
Clonalità
Polyclonal
Isotipo
IgY
Coniugare
Unconjugated
Purificazione
Immunogen affinity purification.
Concentrazione
1 mg/ml
Peso molecolare
190 kDa, 270 kDa, 480 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Ankyrin 3 Antibody (1:2,000 | green), and co-stained with Anti-NeuN Antibody (1:2,000 | red). The blue is Hoechst staining of nuclear DNA. The Anti-Ankyrin 3 Antibody stains the axonal initial segments, while the Anti-NeuN Antibody reveals perikarya and nuclei of neurons.
Figure 2: Western Blot - Anti-Ankyrin 3 Antibody (A104313)
Western blot analysis of different tissue and cell lysates using Anti-Ankyrin 3 Antibody (1:3,000 | green). [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.
