Anti-ATF-4 Anticorpo (A308278)

Name: Anti-ATF-4 Antibody
Brand: Antibodies.com
SKU: A308278
Price: 335.0 USD
Availability: InStock

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$335

Anticorpo policlonale di coniglio contro ATF-4 per WB, IHC e ICC/IF.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-ATF-4 Antibody

Descrizione

Rabbit polyclonal antibody to ATF-4.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 260-351 of human ATF4 (NP_877962.1).

Sequenza

PYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

50 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Activating transcription factor 4, ATF 4, ATF4, ATF4 protein, ATF4_HUMAN, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB 2, CREB-2, CREB2, Cyclic AMP dependent transcription factor ATF 4, Cyclic AMP response element binding protein 2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2, DNA binding protein TAXREB67, DNA-binding protein TAXREB67, Tax Responsive Enhancer Element B67, Tax-responsive enhancer element-binding protein 67, TaxREB67, TXREB

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-ATF-4 Antibody (A308278)

Western blot analysis of extracts of C2C12 cells, using Anti-ATF-4 Antibody (A308278) at 1:500 dilution. C2C12 cells were treated by tunicamycin (2 µg/ml) for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-ATF-4 Antibody (A308278)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ATF-4 Antibody (A308278) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ATF-4 Antibody (A308278)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-ATF-4 Antibody (A308278) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-ATF-4 Antibody (A308278)

Immunofluorescence analysis of human breast cancer cells using Anti-ATF-4 Antibody (A308278) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).