Anti-ATP7A Anticorpo [S60-4] (A304976)

Name: Anti-ATP7A Antibody [S60-4]
Brand: Antibodies.com
SKU: A304976
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S60-4] di topo contro ATP7A per WB, IHC, ICC/IF e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-ATP7A Antibody [S60-4]

Descrizione

Mouse monoclonal [S60-4] antibody to ATP7A.

Specificità

Band detected in rat brain membrane preparations.

Applicazioni

WB, IHC, ICC/IF, IP

Diluizioni consigliate

WB: 1:500, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S60-4

Isotipo

IgG2b

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~180 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

ATP 7A, ATP7A_HUMAN, ATPase copper transporting alpha polypeptide, ATPase Cu++ transporting alpha polypeptide, ATPase Cu++ transporting alpha polypeptide (Menkes syndrome), Copper pump 1, Copper transporting ATPase 1, Copper-transporting ATPase 1, Cu++ transporting P type ATPase, DSMAX, FLJ17790, MC 1, MC1, Menkes disease associated protein, Menkes disease-associated protein, Menkes syndrome, MK, MNK, OHS, OTTHUMP00000062077, SMAX3

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-ATP7A Antibody [S60-4] (A304976)

Western blot analysis of rat liver showing detection of ~180 kDa Copper Transporting ATPase 1 protein using Anti-ATP7A Antibody [S60-4] (A304976) at 1:500 for 2 hours at room temperature with shaking. Lane 1: MW Ladder. Lane 2: rat liver. Load: 10 ug. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~180 kDa.

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Figure 2: Immunofluorescence - Anti-ATP7A Antibody [S60-4] (A304976)

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATP7A Antibody [S60-4] (A304976), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 1 Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-ATP7A Antibody [S60-4] (A304976)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-ATP7A Antibody [S60-4] (A304976), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 1 Antibody (D) Composite.