Anti-Aurora A + B + C Kinase Anticorpo [4A7] (A85401)

Name: Anti-Aurora A + B + C Kinase Antibody [4A7]
Brand: Antibodies.com
SKU: A85401
Price: 185.0 USD
Availability: InStock

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$185

Anticorpo monoclonale [4A7] di topo contro Aurora A per WB, ICC/IF e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

4-6 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Aurora A + B + C Kinase Antibody [4A7]

Descrizione

Mouse monoclonal (4A7) antibody to Aurora A + B + C Kinase.

Specificità

This antibody was raised against human Aurora A protein and was shown to also bind Aurora B and Aurora C; as a result the epitope is likely within the highly conserved serine/threonine kinase domain. This antibody can be used to identify dividing or soon to be dividing cells and is also an excellent marker of midbodies both during and after cell division.

Applicazioni

WB, ICC/IF, IHC

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:1,000-1:2,000, IHC: 1:1,000-1:2,000

Reattività

Human, Horse, Bovine, Porcine, Rat, Mouse

Immunogeno

Recombinant full-length human Aurora A protein, expressed in and purified from E. coli.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

4A7

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Immunogen affinity purification.

Concentrazione

1 mg/ml

Peso molecolare

46kDa, 38kDa, 35kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

AIK, ARK-1, ARK1, AURA, Aurka, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1 like kinase, Aurora/IPL1-related kinase 1, AURORA2, Breast tumor-amplified kinase, BTAK, hARK1, IAK, IPL1 related kinase, MGC34538, OTTHUMP00000031340, OTTHUMP00000031341, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071, OTTHUMP00000166072, PPP1R47, Protein phosphatase 1, regulatory subunit 47, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, STK15, STK6, STK6_HUMAN, STK7

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Immunofluorescence - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

HeLa cell cultures were stained with Anti-Aurora A/B/C Kinase Antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

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Figure 2: Western Blot - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

Left: Western blot analysis of Anti-Aurora A/B/C Kinase Antibody in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Anti-Aurora A/B/C Kinase Antibody. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. The Anti-Aurora A/B/C Kinase Antibody binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with Anti-Aurora A/B/C Kinase Antibody. This antibody reacts strongly with all three Aurora kinases proteins.