Anti-Beclin 1 Anticorpo [ARC52856] (A307234)

Western blot analysis of extracts of various cell lines, using Anti-Beclin 1 Antibody [ARC52856] (A307234) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of Rat lung, using Anti-Beclin 1 Antibody [ARC52856] (A307234) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Western blot analysis of extracts from wild type (WT) and Beclin 1 knockout (KO) 293T cells, using Anti-Beclin 1 Antibody [ARC52856] (A307234) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Confocal imaging of HeLa cells using Anti-Beclin 1 Antibody [ARC52856] (A307234), at a dilution of 1:100, (red). The cells were counterstained with Anti-Vimentin Antibody, at a dilution of 1:100, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.