This antibody recognises cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein.Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. Clone 4C2 is highly specific for cytoplasmic actin ß and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodeling.
Applicazioni
WB, IF, IHC-Fr
Diluizioni consigliate
IHC-Fr: 1:50 - 1:100
Reattività
Human, Rabbit, Pig, Mouse, Rat
Immunogeno
Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to Keyhole Limpet Haemocyanin.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
4C2
Isotipo
IgG1
Coniugare
Unconjugated
Purificazione
Protein A affinity chromatography of tissue culture supernatant.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Conservazione
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Note generali
Mouse anti Human actin beta, clone 4C2, recognizes cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein.Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhove et al. 1978). Clone 4C2 is highly specific for cytoplasmic actin ß and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodeling (Miazza et al. 2011).
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