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Anti-beta Catenin Anticorpo [ARC0136] (A307069)

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Anti-beta Catenin Anticorpo [EM-22]
Anticorpo monoclonale [EM-22] di topo contro beta Catenin per IP, WB, ICC e Flow Cytometry (Intracellular).
Nome
Anti-beta Catenin Antibody [ARC0136]
Descrizione
Rabbit monoclonal [ARC0136] antibody to beta Catenin.
Applicazioni
WB, IHC, ICC/IF, IP
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (P35222).
Sequenza
MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC0136
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
92 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1, 88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1_HUMAN, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, OTTHUMP00000209289
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts of HeLa cells, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts of Mouse brain, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts from wild type (WT) and beta-Catenin knockout (KO) 293T cells, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-beta Catenin Antibody [ARC0136] (A307069) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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