BRCA1 (phospho Ser1524) Cell Based Kit Elisa (A102715)

$645
Spese di spedizione
Tempi di consegna
5-8 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
BRCA1 (phospho Ser1524) Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This BRCA1 (phospho Ser1524) Cell Based ELISA Kit allows for the detection of BRCA1 (phospho Ser1524) and the effects that certain stimulation conditions have on BRCA1 (phospho Ser1524) expression in different cell lines. Qualitative determination of BRCA1 (phospho Ser1524) concentration is achieved by an indirect ELISA format. In essence, the BRCA1 (phospho Ser1524) is captured by Anti-BRCA1 (phospho Ser1524) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BRCA1 (phospho Ser1524) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-BRCA1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
BRCA 1, BRCA1 DNA repair associated, BRCA1/BRCA2 containing complex subunit 1, BRCA1/BRCA2-containing complex, subunit 1, BRCA1_HUMAN, BRCAI, BRCC 1, BRCC1, Breast and ovarian cancer susceptibility protein 1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast cancer type 1 susceptibility protein, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, Protein phosphatase 1 regulatory subunit 53, PSCP, RING finger protein 53, RNF53
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Prodotti alternativi a BRCA1 (phospho Ser1524) Cell Based Kit Elisa (A102715)

Inizio pagina