Anti-CACNA1H Anticorpo [N55/10] (A304750)

Name: Anti-CACNA1H Antibody [N55/10]
Brand: Antibodies.com
SKU: A304750
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [N55/10] di topo contro CACNA1H per WB, IHC, ICC/IF, IP e Antibody Microarray.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-CACNA1H Antibody [N55/10]

Descrizione

Mouse monoclonal [N55/10] antibody to CACNA1H.

Applicazioni

WB, IHC, ICC/IF, IP, Antibody Microarray

Diluizioni consigliate

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Reattività incrociata

This antibody does not cross-react with Cav1.3.

Immunogeno

Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

N55/10

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~260 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Note generali

This antibody was formely sold under the Clone ID S55-10.

Sinonimi

Alpha1 3.2, Alpha13.2, CAC1H_HUMAN, CACNA 1H, CACNA1 H, CACNA1 HB, CACNA1HB, Calcium channel alpha13.2 subunit, Calcium channel voltage dependent T type alpha 1H subunit, Calcium channel, voltage-dependent, T type, alpha 1Hb subunit, Cav 3.2, Cav T.2, Cav3.2, CavT.2, EIG 6, EIG6, Low voltage activated calcium channel alpha 13.2 subunit, Low voltage activated calcium channel alpha1 3.2 subunit, Low-voltage-activated calcium channel alpha1 3.2 subunit, MNCb 1209, T type Cav3.2, Voltage dependent t type calcium channel alpha 1H subunit, Voltage gated calcium channel alpha subunit Cav 3.2, Voltage gated calcium channel alpha subunit Cav T.2, Voltage gated calcium channel alpha subunit Cav3.2, Voltage gated calcium channel alpha subunit CavT.2, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-CACNA1H Antibody [N55/10] (A304750)

Western blot analysis of rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 2 hours at room temperature. Block: 2% Skim Milk + 2% BSA in TBST. The secondary antibody used was Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.

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Figure 2: Immunofluorescence - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CACNA1H Antibody [N55/10] (A304750), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.

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Figure 3: Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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Figure 4: Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.