Anti-Caspase-9 Anticorpo (A14108)

Name: Anti-Caspase-9 Antibody
Brand: Antibodies.com
SKU: A14108

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Anti-Caspase-9 Anticorpo (A14108) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-Caspase-9 Anticorpi.

Descrizione Prodotto

Nome

Anti-Caspase-9 Antibody

Descrizione

Rabbit polyclonal antibody to Caspase-9.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2).

Sequenza

VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

36 kDa / 46 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

APAF-3, APAF3, Apoptosis related cysteine peptidase, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, CASP9_HUMAN, Caspase 9 apoptosis related cysteine peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9 subunit p10, ICE LAP6, ICE like apoptotic protease 6, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, PPP1R56, protein phosphatase 1, regulatory subunit 56, RNCASP9

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-Caspase-9 Antibody (A14108)

Western blot analysis of extracts of various cell lines, using Anti-Caspase-9 Antibody (A14108) at 1:300 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-Caspase-9 Antibody (A14108)

Western blot analysis of extracts of Jurkat cells, using Anti-Caspase-9 Antibody (A14108) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Caspase-9 Antibody (A14108)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Caspase-9 Antibody (A14108) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Caspase-9 Antibody (A14108)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Caspase-9 Antibody (A14108) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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