This Catalase Activity Assay Kit is a quantitative assay kit for measuring Catalase activity in serum, plasma, animal and plant tissues and cells.
Applicazioni
Functional Studies
Principio di kit
This kit provides a simple and easy colorimetric assay for the study of catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. This assay kit utilizes the peroxidase function of catalase for measuring catalase activity.In ther presence of an appropriate concentration of hydrogen peroxide, catalase reacts with methanol to produce formaldehyde which can react with chromogens. The formaldehyde produced can be measured colorimetrically at OD 540 nm.
Tipo di campione
Serum, plasma, animal tissues, plant tissues, and cells.
Componenti
Assay Buffer, Sample Diluent, Formaldehyde Standard (4.25 M), Catalase (Control), Potassium Hydroxide, Hydrogen Peroxide, Chromogen, Potassium Periodate
Conservazione
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Note generali
96-well microplate / microglass cuvette is required.
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Figure 1: Standard Curve - Catalase Activity Assay Kit (A319666)
Representative formaldehyde standard curve for Catalase Activity Assay Kit (A319666). The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (µM).
