Anti-CD3 Anticorpo [CA17.2A12] (A279976)

Anti-CD3 Antibody [CA17.2A12]

Mouse monoclonal [CA17.2A12] antibody to CD3.

This antibody recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin.

Flow Cytometry, IHC-Fr, IP, IF

Flow Cytometry: 1:25 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Canine

Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90.

Mouse

Monoclonal

CA17.2A12

IgG1

Unconjugated

Protein G affinity chromatography of ascites.

1 mg/ml

Liquid

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).

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