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Anti-CD31 Anticorpo [ARC50362] (A305352)

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Anti-CD31 Anticorpo [MEM-05]
Anticorpo monoclonale [MEM-05] di topo contro CD31 per Flow Cytometry, WB, IHC-P e ICC.
Nome
Anti-CD31 Antibody [ARC50362]
Descrizione
Rabbit monoclonal [ARC50362] antibody to CD31.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:500-1:1,000, ICC/IF: 1:100-1:500
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM1 (NP_000433.4).
Sequenza
AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC50362
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
135 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD31 Antibody [ARC50362] (A305352) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD31 Antibody [ARC50362] (A305352)
Western blot analysis of extracts of various cell lines, using Anti-CD31 Antibody [ARC50362] (A305352) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-CD31 Antibody [ARC50362] (A305352) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-CD31 Antibody [ARC50362] (A305352)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD31 Antibody [ARC50362] (A305352) at a dilution of 1:600(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD31 Antibody [ARC50362] (A305352) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-CD31 Antibody [ARC50362] (A305352)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD31 Antibody [ARC50362] (A305352) at a dilution of 1:600(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD31 Antibody [ARC50362] (A305352) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-CD31 Antibody [ARC50362] (A305352)
Immunofluorescence analysis of human placentla using Anti-CD31 Antibody [ARC50362] (A305352) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).

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