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Anti-CD43 Anticorpo (A9855)

Nome
Anti-CD43 Antibody
Descrizione
Rabbit polyclonal antibody to CD43.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 276-400 of human SPN (NP_003114.1).
Sequenza
LWRRRQKRRTGALVLSRGGKRNGVVDAWAGPAQVPEEGAVTVTVGGSGGDKGSGFPDGEGSSRRPTLTTFFGRRKSRQGSLAMEELKSGSGPSLKGEEEPLVASEDGAVDAPAPDEPEGGDGAAP
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
115 - 130 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CD 43, CD43 antigen, Galactoglycoprotein, GALGP, GPL 115, GPL115, Human gene for sialophorin, Leucocyte sialoglycoprotein, Leukocyte large sialoglycoprotein, Leukocyte sialoglycoprotein, Leukosialin, LEUK_HUMAN, LSN, Ly-48, Sialophorin, sialophorin (gpL115, leukosialin, CD43), Spn
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD43 Antibody (A9855) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD43 Antibody (A9855)
Western blot analysis of extracts of various cell lines, using Anti-CD43 Antibody (A9855) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-CD43 Antibody (A9855) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-CD43 Antibody (A9855)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD43 Antibody (A9855) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD43 Antibody (A9855) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-CD43 Antibody (A9855)
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-CD43 Antibody (A9855) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD43 Antibody (A9855) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-CD43 Antibody (A9855)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CD43 Antibody (A9855) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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