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Anti-CD73 Anticorpo (A13821)

Nome
Anti-CD73 Antibody
Descrizione
Rabbit polyclonal antibody to CD73.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 32-264 of human CD73/NT5E (NP_002517.1).
Sequenza
LHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYP
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
70 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
5' NT, 5' nucleotidase (CD73), 5' nucleotidase precursor, 5' nucleotidase, ecto, 5' nucleotidase, ecto (CD73), 5'-NT, 5'-nucleotidase, 5NTD_HUMAN, CD73 antigen, E5NT, Ecto 5' nucleotidase, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD73 Antibody (A13821) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD73 Antibody (A13821)
Western blot analysis of extracts of various cell lines, using Anti-CD73 Antibody (A13821) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-CD73 Antibody (A13821) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-CD73 Antibody (A13821)
Western blot analysis of extracts of Rat eye, using Anti-CD73 Antibody (A13821) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-CD73 Antibody (A13821) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Western Blot - Anti-CD73 Antibody (A13821)
Western blot analysis of extracts from normal (control) and CD73/NT5E Rabbit polyclonal antibody knockout (KO) HCT116 cells, using Anti-CD73 Antibody (A13821) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD73 Antibody (A13821) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-CD73 Antibody (A13821)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD73 Antibody (A13821) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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