This antibody recognises the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine a/ß T cells, in thymic tissue both CD8lo and CD8hi cells are recognized. recognises a different epitope on the CD8 beta chain to clone PPT22.

Applicazioni

Flow Cytometry

Diluizioni consigliate

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Reattività

Porcine

Immunogeno

Porcine thymus membrane lysate.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

PPT23

Isotipo

IgG1

Coniugare

FITC

Excitation: 490nm, Emission: 525nm

Purificazione

Protein A affinity chromatography of tissue culture supernatant.

Concentrazione

100 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conservazione

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Note generali

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine a/ß T cells, in thymic tissue both CD8lo and CD8hi cells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22.

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