Anti-CDK2 (phospho Thr160) Anticorpo [ARC57167] (A309005)

Name: Anti-CDK2 (phospho Thr160) Antibody [ARC57167]
Brand: Antibodies.com
SKU: A309005

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Questo prodotto è fuori produzione

Anti-CDK2 (phospho Thr160) Anticorpo [ARC57167] (A309005) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-CDK2 Anticorpi.

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Anticorpo policlonale di coniglio contro Cdk2 per WB, IF e ELISA.

Descrizione Prodotto

Nome

Anti-CDK2 (phospho Thr160) Antibody [ARC57167]

Descrizione

Rabbit monoclonal [ARC57167] antibody to CDK2 (phospho Thr160).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:2,000-1:6,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around T160 of human Phospho-CDK2-T160 (NP_001789.2).

Sequenza

TYTHE

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC57167

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

34 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Cdc2 related protein kinase, cdc2-related protein kinase, CDC28, CDC2A, Cdk 2, CDK1, CDK2_HUMAN, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, cyclin dependent kinase 2-alpha, Cyclin-dependent kinase 2, kinase Cdc2, MPF, p33 protein kinase, p33(CDK2)

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Western blot analysis of HeLa, using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at 1:5,000 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Western blot analysis of C6, using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at 1:5,000 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.