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Anti-CEACAM5 Anticorpo [AMC0141] (A306438)

Nome
Anti-CEACAM5 Antibody [AMC0141]
Descrizione
Mouse monoclonal [AMC0141] antibody to CEACAM5.
Applicazioni
WB, IHC, IP
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:50-1:200
Reattività
Human
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 35-685 of human CEACAM5.
Sequenza
KLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSA
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
AMC0141
Isotipo
IgG1
Catene leggere
kappa
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
200 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Carcinoembryonic antigen, Carcinoembryonic antigen related cell adhesion molecule 5, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CD66e antigen, CEA, CEAM5_HUMAN, Meconium antigen 100
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CEACAM5 Antibody [AMC0141] (A306438) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CEACAM5 Antibody [AMC0141] (A306438)
Western blot analysis of extracts of MCF7 cells, using Anti-CEACAM5 Antibody [AMC0141] (A306438) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-CEACAM5 Antibody [AMC0141] (A306438) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-CEACAM5 Antibody [AMC0141] (A306438)
Immunohistochemistry analysis of paraffin-embedded human tonsil (negative control sample) using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CEACAM5 Antibody [AMC0141] (A306438) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-CEACAM5 Antibody [AMC0141] (A306438)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CEACAM5 Antibody [AMC0141] (A306438) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-CEACAM5 Antibody [AMC0141] (A306438)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CEACAM5 Antibody [AMC0141] (A306438) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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