This antibody recognises human CNTF (ciliary neurotrophic factor), a 200 amino acid homodimeric neurotrophic and differentiation factor of the CNTF family, which supports the survival and differentiation of several motor neuron types within the nervous system, but also affects other target tissues, including skeletal muscle , glial cells, hepatocytes and embryonic stem cells.CNTF mainly signals through binding to a receptor complex consisting of a CNTFRa subunit, two beta components, gp130 and LIFR, resulting ultimately in the activation of JAK/STAT signalling pathway, but CNTF has also been shown to bind to the receptor for interleukin-6 (IL-6R).

Applicazioni

WB, ELISA, IHC-P, Functional Assays

Diluizioni consigliate

ELISA: 0.5 - 2 µg/ml, Functional Assays: 7.0 - 11 µg/ml, IHC-P: 0.25 µg/ml, WB: 0.1 - 0.2 µg/ml

Reattività

Human

Immunogeno

Recombinant human CNTF.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Concentrazione

1 mg/ml after reconstitution.

Forma del prodotto

Lyophilized

Ricostituzione

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide

Formulazione

Supplied in Phosphate Buffered Saline.

Conservazione

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Note generali

Rabbit anti Human CTNF antibody recognizes human CNTF (ciliary neurotrophic factor), a 200 amino acid homodimeric neurotrophic and differentiation factor of the CNTF family, which supports the survival and differentiation of several motor neuron types within the nervous system, but also affects other target tissues, including skeletal muscle (Vergara & Ramirez 2005), glial cells, hepatocytes and embryonic stem cells.CNTF mainly signals through binding to a receptor complex consisting of a CNTFRa subunit, two beta components, gp130 and LIFR, resulting ultimately in the activation of JAK/STAT signalling pathway, but CNTF has also been shown to bind to the receptor for interleukin-6 (IL-6R).

Sinonimi

AI429687, Ciliary Neurotrophic Factor, CNTF_HUMAN, HCNTF, MGC41235, OTTHUMP00000174731, OTTMUSP00000029848

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.