Anti-CREB (phospho Ser133) Anticorpo (A10868)

Name: Anti-CREB (phospho Ser133) Antibody
Brand: Antibodies.com
SKU: A10868
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro CREB (phospho Ser133) per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-CREB (phospho Ser133) Antibody

Descrizione

Rabbit polyclonal antibody to CREB (phospho Ser133).

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:200-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).

Sequenza

RPSYR

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

43 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Active transcription factor CREB, cAMP response element binding protein, cAMP response element binding protein 1, cAMP responsive element binding protein 1, cAMP-responsive element-binding protein 1, CREB-1, CREB1, CREB1_HUMAN, Cyclic AMP-responsive element-binding protein 1, MGC9284, OTTHUMP00000163864, OTTHUMP00000163865, OTTHUMP00000206660, OTTHUMP00000206662, OTTHUMP00000206667, Transactivator protein

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-CREB (phospho Ser133) Antibody (A10868)

Western blot analysis of extracts of HeLa cells, using Anti-CREB (phospho Ser133) Antibody (A10868) at 1:2,000 dilution or CREB1 antibody (A11064). HeLa cells were treated by Anisomycin (25 ug/mL) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10868)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CREB (phospho Ser133) Antibody (A10868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10868)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-CREB (phospho Ser133) Antibody (A10868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10868)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-CREB (phospho Ser133) Antibody (A10868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.