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Anti-Cytokeratin 19 Anticorpo (A12571)

Nome
Anti-Cytokeratin 19 Antibody
Descrizione
Rabbit polyclonal antibody to Cytokeratin 19.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 241-400 of human Cytokeratin 19 (Cytokeratin 19 (KRT19)) (NP_002267.2).
Sequenza
PGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
40 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
40 kDa keratin intermediate filament, CK 19, CK-19, CK19, Cytokeratin-19, K19, K1C19_HUMAN, K1CS, Keratin 19, Keratin type I 40 kD, Keratin type I 40kD, Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, Keratin, type I, 40 kd, Keratin-19, KRT19, MGC15366
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Cytokeratin 19 Antibody (A12571) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Cytokeratin 19 Antibody (A12571)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 19 Antibody (A12571) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody (A12571) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-Cytokeratin 19 Antibody (A12571)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Cytokeratin 19 Antibody (A12571) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody (A12571) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Cytokeratin 19 Antibody (A12571)
Immunohistochemistry analysis of paraffin-embedded human liver (negative control sample) using Anti-Cytokeratin 19 Antibody (A12571) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody (A12571) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-Cytokeratin 19 Antibody (A12571)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Cytokeratin 19 Antibody (A12571) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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