Figure 1: Western Blot - Anti-DDDDK Tag Antibody (A17310)
Western blot analysis of 293T, using Anti-DDDDK Tag Antibody (A17310) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-DDDDK Tag Antibody (A17310)
Western blot analysis of 293T, using Anti-DDDDK Tag Antibody (A17310) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 3: Immunofluorescence - Anti-DDDDK Tag Antibody (A17310)
Immunofluorescence analysis of 293T cells using Anti-DDDDK Tag Antibody (A17310) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 4: Immunofluorescence - Anti-DDDDK Tag Antibody (A17310)
Immunofluorescence analysis of 293T-BCAT2-Flag-GFP-C cells using Anti-DDDDK Tag Antibody (A17310) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 5: Immunofluorescence - Anti-DDDDK Tag Antibody (A17310)
Immunofluorescence analysis of 293T-BCAT2-Flag-GFP-N cells using Anti-DDDDK Tag Antibody (A17310) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 6: Western Blot - Anti-DDDDK Tag Antibody (A17310)
Immunoprecipitation analysis of 200µg extract cell lysate from 293T cells transfected with GSK3B expression vector containing a DDDDK-Tag using 3µg of Anti-DDDDK Tag Antibody (A17310). This Western blot was performed on the immunoprecipitate using Anti-DDDDK Tag Antibody (A17310).
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