Anti-DENND4C Anticorpo [3E8] (A304978)

Western blot analysis of human A549, MEF cells showing detection of DENND4C protein using Anti-DENND4C Antibody [3E8] (A304978) at 1:1,000. Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug.

Immunoprecipitation analysis of human, mouse A549, MEF cells using Anti-DENND4C Antibody [3E8] (A304978). Three amounts of A304978 (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C.

Immunocytochemistry/Immunofluorescence analysis of human A549 cells, using Anti-DENND4C Antibody [3E8] (A304978), at 1:100, 1:500, 1:1,000 for 1 hour at room temperature. The secondary antibody used was Donkey anti-mouse: Alexa Fluor 594 at 1:1,000 for 1 hour at room temperature. Counterstain: DAPI.