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Anti-Dnmt1 Anticorpo [ARC51345] (A307588)

Nome
Anti-Dnmt1 Antibody [ARC51345]
Descrizione
Rabbit monoclonal [ARC51345] antibody to Dnmt1.
Applicazioni
WB, IHC, IP
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_001370.1).
Sequenza
MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYN
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC51345
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
200 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Dnmt1 Antibody [ARC51345] (A307588) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Dnmt1 Antibody [ARC51345] (A307588)
Western blot analysis of various lysates, using Anti-Dnmt1 Antibody [ARC51345] (A307588) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Dnmt1 Antibody [ARC51345] (A307588) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Dnmt1 Antibody [ARC51345] (A307588)
Western blot analysis of various lysates, using Anti-Dnmt1 Antibody [ARC51345] (A307588) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-Dnmt1 Antibody [ARC51345] (A307588) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Dnmt1 Antibody [ARC51345] (A307588)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Dnmt1 Antibody [ARC51345] (A307588) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Dnmt1 Antibody [ARC51345] (A307588) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-Dnmt1 Antibody [ARC51345] (A307588)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Dnmt1 Antibody [ARC51345] (A307588) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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