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Anti-EIF3D Anticorpo (A80439)

Nome
Anti-EIF3D Antibody
Descrizione
Rabbit polyclonal antibody to EIF3D.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:200-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).
Sequenza
MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYAYFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKFQKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRITTRSEKPLRSIKRIFHTVTTTDDPVIRKLAK
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
64 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
eIF 3 zeta, eIF-3-zeta, eIF3 p66, eIF3 zeta, EIF3D_HUMAN, eIF3p66, EIF3S7, Eukaryotic translation initiation factor 3 subunit 7, Eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kDa, Eukaryotic translation initiation factor 3 subunit D, MGC126526, MGC17258, OTTHUMP00000197900, OTTHUMP00000197901, Translation initiation factor eIF3 p66 subunit
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-EIF3D Antibody (A80439) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-EIF3D Antibody (A80439)
Western blot analysis of extracts of various cell lines, using Anti-EIF3D Antibody (A80439) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-EIF3D Antibody (A80439) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunofluorescence - Anti-EIF3D Antibody (A80439)
Immunofluorescence analysis of L-929 cells using Anti-EIF3D Antibody (A80439) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-EIF3D Antibody (A80439) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-EIF3D Antibody (A80439)
Immunofluorescence analysis of C6 cells using Anti-EIF3D Antibody (A80439) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-EIF3D Antibody (A80439) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-EIF3D Antibody (A80439)
Immunofluorescence analysis of L-929 cells using Anti-EIF3D Antibody (A80439) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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