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Anti-ENPP5 Anticorpo [ARC2780] (A305501)

Nome
Anti-ENPP5 Antibody [ARC2780]
Descrizione
Rabbit monoclonal [ARC2780] antibody to ENPP5.
Applicazioni
WB, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 378-477 of human ENPP5 (Q9UJA9).
Sequenza
CHLLNITAMPHNGSFWNVQDLLNSAMPRVVPYTQSTILLPGSVKPAEYDQEGSYPYFIGVSLGSIIVIVFFVIFIKHLIHSQIPALQDMHAEIAQPLLQA
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC2780
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
54 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
E-NPP 5, E-NPP5, Ectonucleotide pyrophosphatase/phosphodiesterase 5, ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function), Ectonucleotide pyrophosphatase/phosphodiesterase family member 5, ENPP5_HUMAN, NPP-5, UNQ550/PRO1107
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501)
Western blot analysis of extracts of various cell lines, using Anti-ENPP5 Antibody [ARC2780] (A305501) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501)
Western blot analysis of extracts of various cell lines, using Anti-ENPP5 Antibody [ARC2780] (A305501) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501)
Immunofluorescence analysis of Neuro-2a cells using Anti-ENPP5 Antibody [ARC2780] (A305501) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501)
Immunofluorescence analysis of SH-SY5Y cells using Anti-ENPP5 Antibody [ARC2780] (A305501) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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