Figure 1: Western Blot - Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716)
Western blot analysis of various lysates, using Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Figure 2: Western Blot - Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716)
Western blot analysis of various lysates, using Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence analysis of mouse spleen using Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat stomach using Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 5: Western Blot - Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716)
Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716). This Western blot was performed on the immunoprecipitate using Anti-Estrogen Inducible Protein pS2 Antibody [ARC59782] (A309716) at a dilution of 1:1000.