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Anti-Fascin Anticorpo (A13733)

Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.com Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.com Immunohistochemistry - Anti-Fascin Antibody (A13733) - Antibodies.com Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.com Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.com
Knockout Convalidato
$470
Product Datasheet

Anticorpo policlonale di coniglio contro Fascin per WB, IHC e ICC/IF.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Fascin Antibody
Descrizione
Rabbit polyclonal antibody to Fascin.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human Fascin/Fascin/FSCN1 (NP_003079.1).
Sequenza
FIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
50 kDa / 55 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
55 kDa actin bundling protein, 55 kDa actin-bundling protein, Actin bundling protein, actin bundling protein, 55-KD, FAN 1, FAN1, Fascin 1, Fascin actin bundling protein 1, Fascin homolog 1, Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus), Fascin, sea urchin, homolog of, 1, Fascin1, FLJ38511, FSCN 1, FSCN1, FSCN1_HUMAN, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), Singed drosophila homolog like, Singed like (fascin homolog sea urchin), Singed like (fascin homolog sea urchin) (Drosophila), Singed like protein, Singed, drosophila, homolog of, Singed-like protein, SNL, Strongylocentrotus purpuratus
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Fascin Antibody (A13733)
Western blot analysis of extracts of various cell lines, using Anti-Fascin Antibody (A13733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Fascin Antibody (A13733)
Western blot analysis of extracts from normal (control) and Fascin/Fascin/FSCN1 knockout (KO) HeLa cells, using Anti-Fascin Antibody (A13733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-Fascin Antibody (A13733) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Fascin Antibody (A13733)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Fascin Antibody (A13733) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-Fascin Antibody (A13733)
Immunofluorescence analysis of C6 cells using Anti-Fascin Antibody (A13733) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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