Anti-Fatty Acid Synthase Anticorpo [ARC53122] (A308364)

Name: Anti-Fatty Acid Synthase Antibody [ARC53122]
Brand: Antibodies.com
SKU: A308364

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Questo prodotto è fuori produzione

Anti-Fatty Acid Synthase Anticorpo [ARC53122] (A308364) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-Fatty Acid Synthase Anticorpi.

Descrizione Prodotto

Nome

Anti-Fatty Acid Synthase Antibody [ARC53122]

Descrizione

Rabbit monoclonal [ARC53122] antibody to Fatty Acid Synthase.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:500-1:1,000, ICC/IF: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).

Sequenza

QQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC53122

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

273 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Enoyl-[acyl-carrier-protein] reductase, FAS, FASN, FAS_HUMAN, MGC14367, MGC15706, OA 519, Oleoyl-[acyl-carrier-protein] hydrolase, SDR27X1, Short chain dehydrogenase/reductase family 27X member 1, [Acyl-carrier-protein] S acetyltransferase, [Acyl-carrier-protein] S malonyltransferase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts of 293F cells, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts of Rat liver, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts from wild type (WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 4: Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Confocal imaging of HeLa cells using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.