Figure 1: Western Blot - Anti-Glutaredoxin 1 Antibody (A14662)
Western blot analysis of extracts of various cell lines, using Anti-Glutaredoxin 1 Antibody (A14662) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Glutaredoxin 1 Antibody (A14662) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human kidney cancer using Anti-Glutaredoxin 1 Antibody (A14662) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Glutaredoxin 1 Antibody (A14662) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-Glutaredoxin 1 Antibody (A14662) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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