Figure 1: Western Blot - Anti-GSK3 beta (phospho Ser9) Antibody (A10877)
Western blot analysis of C6, using Anti-GSK3 beta (phospho Ser9) Antibody (A10877) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-GSK3 beta (phospho Ser9) Antibody (A10877) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-GSK3 beta (phospho Ser9) Antibody (A10877) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 4: Western Blot - Anti-GSK3 beta (phospho Ser9) Antibody (A10877)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-GSK3 beta (phospho Ser9) Antibody (A10877). This Western blot was performed on the immunoprecipitate using Anti-GSK3 beta (phospho Ser9) Antibody (A10877) at a dilution of 1:1000.
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