This antibody reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
Applicazioni
WB
Diluizioni consigliate
WB: 1-2 µg/ml; Reducing conditions.
Reattività
Human
Immunogeno
Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.
Sequenza
DIHQYREQIKRVKDSDDC
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
H-RAS-03
Isotipo
IgG1
Coniugare
Unconjugated
Purificazione
Protein A chromatography.
Concentrazione
1 mg/ml
MW previsto
21 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Figure 1: Western Blot - Anti-H-RAS Antibody [H-RAS-03] (A86021)
Western Blotting analysis (reducing conditions) of H-Ras in whole cell lysate using Anti-H-Ras Antibody (A86021). Lane 1: HeLa human cervix carcinoma cell line. Lane 2: K567 human leukemia cell line. Lane 3: RAJI human Burkitt lymphoma cell line.