Anti-Histone H3.3 Anticorpo [ARC0255] (A306483)

Name: Anti-Histone H3.3 Antibody [ARC0255]
Brand: Antibodies.com
SKU: A306483
Price: 470.0 USD
Availability: InStock

Clicca sull'immagine per la visualizzazione estesa

$470

Anticorpo monoclonale [ARC0255] di coniglio contro Histone H3.3 per WB, IHC, IP, ChIP e ChIP-seq.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Descrizione Prodotto

Nome

Anti-Histone H3.3 Antibody [ARC0255]

Descrizione

Rabbit monoclonal [ARC0255] antibody to Histone H3.3.

Applicazioni

WB, IHC, IP, ChIP, ChIP-seq

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, ChIP-seq: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 57-136 of human Histone H3.3 (P84243).

Sequenza

KSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0255

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

17 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

H3 histone family 3A, H3 histone family 3B, H3 histone, family 3B (H3.3B), H3.3, H3.3A, H3.3B, H33_HUMAN, H3F3, H3F3A, H3f3b, Histone H3.3Q, Histone H3.A, Histone H3.B, MGC87782, MGC87783

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (8)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Chromatin Immunoprecipitation - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Chromatin immunoprecipitations analysis of cross-linked chromatin from HeLa cells, using Anti-Histone H3.3 Antibody [ARC0255] (A306483). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Western blot analysis of extracts of Rat lung, using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Western Blot - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Immunohistochemistry - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.