Anti-HSF1 Anticorpo [10H4] (A305102)

Name: Anti-HSF1 Antibody [10H4]
Brand: Antibodies.com
SKU: A305102
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [10H4] di ratto contro HSF1 per WB, IHC, ICC/IF, IP, ELISA e Gel Shift.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-HSF1 Antibody [10H4]

Descrizione

Rat monoclonal [10H4] antibody to HSF1.

Specificità

Bands correspond to unstressed cell lysates and heat shocked cell lysates.

Applicazioni

WB, IHC, ICC/IF, IP, ELISA, Gel Shift

Diluizioni consigliate

WB: 1:1,000, IHC: 1:8,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

Immunogeno

Purified recombinant mouse HSF1 protein.

Specie ospite

Rat

Clonalità

Monoclonal

Clona ID

10H4

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~85 kDa / ~95 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, HSF1_HUMAN, HSTF 1, HSTF1

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Immunohistochemistry - Anti-HSF1 Antibody [10H4] (A305102)

Immunohistochemistry analysis of human breast carcinoma, fixed in 10% formalin solution for 20 hours at room temperature. The Primary Antibody used was Anti-HSF1 Antibody [10H4] (A305102) at 1:8000 for 40 min. The secondary antibody used was Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 minutes at room temperature. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at room temperature. Localization: Nuclear. Magnification: 100X.

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Figure 2: Immunofluorescence - Anti-HSF1 Antibody [10H4] (A305102)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [10H4] (A305102), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rat (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 3: Western Blot - Anti-HSF1 Antibody [10H4] (A305102)

Western blot analysis of human A431 and HEK293 cell lysates showing detection of HSF1 protein using Anti-HSF1 Antibody [10H4] (A305102) at 1:1,000.

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Figure 4: Immunofluorescence - Anti-HSF1 Antibody [10H4] (A305102)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [10H4] (A305102), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.