Human CCL18 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CCL18 in the samples. A monoclonal antibody specific for human CCL18 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CCL18, and CCL18 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL18 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

3.00 pg/ml

Campo di rilevamento

15.63 - 1000 pg/ml

Specificità

This kit can assay both natural and recombinant human CCL18.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

Alternative macrophage activation associated CC chemokine 1, Alternative macrophage activation-associated CC chemokine 1, AMAC-1, AMAC1, CC chemokine PARC, CCL18(4-69), CCL18_HUMAN, CKb7, DC-CK1, DCCK1, Dendritic cell chemokine 1, Macrophage Inflammatory Protein 4, MIP-4, MIP4, PARC, Pulmonary and activation regulated chemokine, Pulmonary and activation-regulated chemokine, SCYA18, Small inducible cytokine A18, Small-inducible cytokine A18

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.