Human sCD163 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sCD163 in the samples. A monoclonal antibody specific for human sCD163 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sCD163, and sCD163 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sCD163 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

4.39 pg/ml

Campo di rilevamento

62.50 - 4000 pg/ml

Specificità

This kit can assay both natural and recombinant human sCD163.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

C163A_HUMAN, CD 163, CD163 antigen, CD163 molecule, Hemoglobin scavenger receptor, M130, M130 antigen precursor, Macrophage associated antigen, MM130, OTTHUMP00000238617, OTTHUMP00000238618, OTTHUMP00000238619, OTTHUMP00000238620, SCARI1, Scavenger receptor cysteine rich type 1 protein M130, sCD163, Soluble CD163

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.