Umano GM-CSF Kit Elisa (A578)

$635
Spese di spedizione
Tempi di consegna
7-10 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
Human GM-CSF ELISA Kit
Tipo di kit
Sandwich (quantitative)
Principio di kit
This GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-GM-CSF Antibody. Standards or samples are then added to the microtire plate wells and GM-CSF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of GM-CSF present in the sample, Anti-GM-CSF Antibody (Biotin) is added to each well to "sandwich" the GM-CSF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GM-CSF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensibilità
< 1 pg/ml
Campo di rilevamento
15.6-1000 pg/ml
Reattività
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at 2-8°C for 4 months or at -20°C for 8 months.
Sinonimi
Colony stimulating factor, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF 2, CSF2, CSF2_HUMAN, GMCSF, Granulocyte Macrophage Colony Stimulating Factor, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, MGI1GM, Molgramostin, Pluripoietin-a, Sargramostim
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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