Human MMP-13 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human MMP-13 in the samples. A monoclonal antibody specific for human MMP-13 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for MMP-13, and MMP-13 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MMP-13 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Cell culture supernatant, serum and plasma.
Sensibilità
0.43 pg/ml
Campo di rilevamento
31.25 - 2000 pg/ml
Recupero
100% (Average Recovery from Serum Samples).
Specificità
This kit can assay both natural and recombinant human MMP-13.
Reattività
Human
Reattività incrociata
This assay has shown no significant cross-reactivity.
